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USE: ,Class II Biological Safety, Cabinets (BSC's) provide personnel, product, and environmental protection. ,Class II, BSC's must be used for all activities involving low or moderate risk biohazardous agents, etiologic agents, oncogenic viruses, and recombinant DNA experiments requiring ,Biosafety, Level 1, 2, and 3 containment.
Class, 1: ,Class, 2: ,Class, 3: ,Class, 4 : OSHA/EPA Level A PPE Equivalent NFPA 1991 (2005 ed.) ",Class, 1" PPE ensemble worn with NIOSH CBRN SCBA: OSHA/EPA Level B PPE Equivalent NFPA 1994 (2007 ed.) ",Class, 2" PPE ensemble worn with NIOSH CBRN SCBA OR NFPA 1971 (2007 ed.) "CBRN option" PPE ensemble worn with NIOSH CBRN SCBA OSHA/EPA Level C PPE Equivalent
Table 11-1: Summary Table of Key Characteristics ,of Class II Biological Safety, Cabinets (BSCs) Type A1 Type A2 Type B1 Type B2; Minimum average inflow velocity through front opening : 0.38 m/s [75 fpm] 0.51 m/s [100 fpm] 0.51 m/s [100 fpm] 0.51 m/s [100 fpm] Air patterns: 30% of the air is exhausted out of the BSC and 70% of the air is ...
Class II biological safety, cabinets 53 ,Class, III ,biological safety, cabinet 56 ... Personal ,protective, equipment and ,clothing, 64 PART IV. Good microbiological techniques 67 12. Laboratory techniques 69 Safe handling of specimens in the laboratory 69 Use of pipettes and pipetting aids 70
All manipulations of infectious materials are conducted in a ,Class II biosafety, cabinet. Refer to ",Biological Safety, Cabinet" Section ,II,.D of this manual for information on classification and use of ,biosafety, cabinets. ,Wear protective, laboratory ,clothing, such as solid-front or wrap-around gowns, scrub suits, or coveralls when in the laboratory.
All procedures involving the manipulation of infectious materials must be conducted within a ,biological safety, cabinet (BSC) (preferably ,Class II, or ,Class, III) or other physical containment devices. Workers in the laboratory must ,wear protective, laboratory ,clothing, with a solid-front, such as tie-back or wrap-around gowns, scrub suits or coveralls.
There are 3 main classes of cabinets (I, ,II,, III) which provide various levels of protection. ,Class II, and III BSC contain HEPA filters which remove particles (min 0.3 microns) from supply and exhaust air with 99.97 efficiency . BSC should be located away from doors and high traffic areas; 52 ,BIOLOGICAL SAFETY, …
All procedures conducted in a BSL3 laboratory that involves the handling of infectious material must be conducted using a ,Class II,/,Class, III ,biosafety, cabinet. 2. Person ,protective, equipment (PPE) includes a solid front with a tie-back laboratory attire or wrap around gowns, scrub suits or coveralls.
Visitors should be given ,protective clothing, and safety instructions as necessary. Cleaners and service engineers must only be allowed to enter if safe to do so. Control of access to CL2 facilities for maintenance and service personnel should be through a permit to work system via the ,Biological Safety, Officer, Departmental Safety Office or Bioengineering Technician.
20/8/2020, · ,Biosafety, Level 2 (BSL-2) is suitable for experiments involving agents of moderate potential hazard to personnel and the environment. For example: Microorganisms of low biohazard potential, such as those in Risk Group 2 or BSL-2.